Dilution Ratio Calculator

Read it as parts of concentrate to parts of diluent or total. In lab notation, 1:10 means 1 part of sample in 10 parts total, a factor of 10. On many cleaning and photo chemical labels, 1+10 means 1 part concentrate plus 10 parts water, a factor of 11. When unsure, check the label or protocol.

The dilution factor is the final volume divided by the volume of the original solution that went into it, DF = V final / V stock. A DF of 20 means the solution is one twentieth as strong. It also equals C stock / C final, so 100 mg/mL diluted to 5 mg/mL is a factor of 20.

Divide the final volume by the stock volume, or the stock concentration by the final concentration. If 25 mL of concentrate is made up to 500 mL, DF = 500 / 25 = 20. With a stock of 100 mg/mL and a target of 5 mg/mL, DF = 100 / 5 = 20 as well.

One part of the stock goes into 10 parts total, so the final strength is one tenth, 10 percent. For 1,000 mL of finished solution, use 100 mL of stock and 900 mL of diluent. The concentration drops by a factor of 10, for example 100 mg/mL becomes 10 mg/mL.

One part stock in 20 parts total, so the final solution is 5 percent of the original strength. Per litre, that is 50 mL of stock plus 950 mL of diluent. A 100 unit stock becomes 5 units. In the 1+20 convention, the factor would be 21.

Writing 1/200 means the final solution is one two-hundredth as strong as the stock, which is a dilution factor of 200. It is 0.5 percent of the original strength. For 1 litre, use 5 mL of stock and 995 mL of diluent. Antibody and reagent protocols often use fractions like this.

Divide the amounts. With 25 mL of concentrate and 475 mL of diluent, the total is 500 mL, so the factor is 500 / 25 = 20, written 1:20 (lab) or 1+19 (parts plus). The ratio of diluent to concentrate is 475 / 25 = 19. Enter both volumes in the ratio mode.

1:10 puts 1 part in 10 total, so the factor is 10 and 1 L needs 100 mL concentrate. 1+10 adds 10 parts to 1 part, a factor of 11, and 1 L needs 90.9 mL. The difference is about 9 percent, which matters for chemicals. The notation table compares both side by side.

C1 and V1 are the stock concentration and volume, C2 and V2 the final ones. Fill in three values and solve for the fourth. To make 500 mL at 5 mg/mL from 100 mg/mL, V1 = 5 × 500 / 100 = 25 mL, then add 475 mL of diluent. Keep the same units on both sides.

Divide the final volume by the dilution factor. For 3 US gallons at 1+10, the factor is 11, so concentrate = 3 × 3,785 mL / 11 = 1,032 mL, about 34.9 fl oz, and the rest is water. Always follow the product label for real products.

Divide the concentration in g per litre by the molar mass in g per mole to get mol per litre. For 10 mg/mL of sodium chloride, 10 g per L / 58.44 g per mol = 0.1711 M, or 171.1 mM. mg/mL equals g/L.

Divide the mass of powder by the concentration you want. For 50 mg at 10 mg/mL, the volume is 50 / 10 = 5 mL of solvent. Use the reconstitution mode, and add a molar mass to see the molarity as well. Follow the supplier's instructions for the solvent and storage.

It applies C1 x V1 = C2 x V2 with both concentrations in mg/mL. Choose which of C1, V1, C2 and V2 to solve for and enter the other three. Any concentration unit works if both sides use the same one. It also shows the diluent to add and the dilution factor.

It does the arithmetic of concentration and volume, nothing more. It is not for dosing and it does not know what is safe. For any medicine, rely on the pharmacist, prescriber or product instructions. The calculator is meant for laboratory solutions, cleaning products and learning.

Each step dilutes by the same factor, so n steps of factor f give fⁿ overall. Six 10-fold steps give 1,000,000. For tubes of 10 mL, transfer 1 mL and add 9 mL of diluent each time. A start of 1,000 units per mL ends at 0.001 units per mL.

Use the dilution factor from the protocol. A 1:1000 dilution in 10 mL needs 10 mL / 1,000 = 10 microlitres of antibody and the rest buffer, 9.99 mL. Follow the supplier's datasheet for the starting range and the diluent.

Multiply the measured concentration by the dilution factor. If a 1:10 dilution of a cell suspension reads 2.5 × 10⁶ cells per mL, the original is 2.5 × 10⁷ per mL. To dilute to a target, use C1 x V1 = C2 x V2 with the cell counts per mL as the concentrations.

Use C1 x V1 = C2 x V2 with molarity on both sides. To make 100 mL of 0.1 M from a 1 M stock, V1 = 0.1 × 100 / 1 = 10 mL, then add 90 mL of solvent. The same arithmetic works for mM, uM or any other unit if both concentrations match.

Use C1 x V1 = C2 x V2 with ppm as the unit. From a 10,000 ppm stock to 200 ppm in 1 litre, V1 = 200 × 1 / 10,000 = 0.02 L, or 20 mL, with 980 mL of water. The dilution factor is 50.

Check the label for the ratio and whether it is 1:N or 1+N. For 1+10 in one US gallon, use 11.6 fl oz of concentrate and 116.4 fl oz of water. Never mix different products unless the label says so, and wear the protective gear it lists.

The arithmetic is exact for the numbers you enter. Real results depend on measuring accurately, on volumes that are not perfectly additive and on the purity of the stock. These are estimates for education and planning. Follow product labels and safety data sheets, and for pesticides or medicines follow the label or a professional.